Diagnostic value of reverse transcription-PCR of amniotic fluid for prenatal diagnosis of congenital rubella infection in pregnant women with confirmed primary rubella infection.

Confirmed Primary Rubella Infection Infection in Pregnant Women with Prenatal Diagnosis of Congenital Rubella Transcription-PCR of Amniotic Fluid for Diagnostic Value of Reverse

PCR for detection of rubella virus RNA in clinical samples.

A reverse transcription nested PCR (RT-PCR) assay for the detection of rubella virus RNA using primers from the E1 open reading frame was established. This assay was found to be sensitive (detecting approximately two synthetic RNA copies and RNA extracted from 0.1 50% tissue culture infective dose of rubella virus) and specific; five wild-type rubella strains and four vaccine strains were detec...

Rubella Immunity in Pregnant Iranian Women: A Systematic Review and Meta-Analysis

Background: Rubella infection with in the first trimester of pregnancy may lead to adverse pregnancy outcomes. The present study was conducted to evaluate the immunity against rubella among pregnant Iranian women. Materials and Methods: The steps of meta-analyses were conducted based on the PRISMA guidelines. To review the associated English and Persian literature, a comprehensive search was co...

بررسی سرولوژی ویروس سرخجه در زنان داوطلب ازدواج مراجعه کننده به مرکز بهداشت شهرستان همدان

Introduction & Objective: Despite mild and self limited manifestation of rubella , involvement of fetus as a consequence of prenatal infection is very important and can lead to congenital rubella syndrome (Cs). The aim of this study was the evaluation of immunity rate against rubella virus in women who were in child bearing ages. Materials & Methods: This survey was performed with cooperatio...

Prevalence of Rubella and HCMV Antibodies among Neonates with Congenital Defects in Four Provinces of Iran

Background and Aims: Primary infections of rubella and human cytomegalovirus (HCMV) can lead to severe complications in pregnancy. The screening of Rubella and HCMV in pregnant women is not routinely carried out in Iran. This cross sectional study aimed to investigate the seroprevalence of HCMV and rubella infections in neonates with and without congenital defects. Materials and Methods: This s...